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Isn't it a cynical world we now live in where the simplest gestures have now become irrelevant, like little kisses just because, holding hands no matter where you are, opening a door for a woman, or buying flowers for no reason or any reason at all? I believe that it's through patience and not by serendipitous happenstance is how we find that one person you're meant to grow old with, the one person you grow with spiritually and emotionally. My definition of a soul mate is someone who you are so enamored with that every time you breathe, you take them in, every minute away seems like a day, and they are the last person you see as you fall asleep in each others arms. That person would be with you through the hard times and through the best times. And would have the fortitude to say if you open your heart to me, I will open mine to you.
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18.07.2020
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The discovery of such cells in the adult mammalian central nervous system CNS , an organ traditionally thought to have little or no regenerative capacity, opened the door to treatment of degenerative diseases of CNS like Stroke, Parkinson, Alzheimer and Spinal Cord Injury. Thus, here we described the isolation of neural stem cells from the adult mouse brain using the neurosphere assay NSA and differentiation of these cells to neural adult cells in details. Methods: The rostral part of the subventricular zone SVZ of the lateral ventricles in the adult mice was dissociated into single cell suspension and cultured using NSA. Primary neurospheres were counted seven days after plating and then the mean number of neurospheres was recorded. The differentiation of neural stem cells into adult neural cells was accomplished by plating the neurosphere-derived cells in differentiating media. Immunocytochemistry and specific markers were used for the identification of the adult neural stem cells. Results : The cell suspension obtained from the r ostral part of the SVZ of the lateral ventricles generated multipotential colonies, called neurospheres, 7 to 10 days post- incubation.
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